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GrainGenes Marker Report: Fr-A2

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Locus
Fr-A2
Type
Trait
Chromosome
5A
Chromosome Arm
5A
Map
Wheat-Composite2004-5A36
[ Show Nearby Loci ]
Species
Triticum aestivum
Probe
BCD508
S2269949
Associated Gene
Fr-A2 (Triticum)
Homology
T22388e-value: 4.6hypothetical protein F48F7.4 - Caenorhabditis elegans this gene~cDNA EST yk50c1.3 comes from this gene~cDNA EST yk50c1.5 comes from this gene~cDNA EST yk167b2.3 comes from this gene~cDNA EST yk167b2.5 comes from this gene~cDNA EST yk145e8.3>
Gene Class
Frost resistance
Reference
ReferenceVagujfalvi A et al. (2003) The cold-regulated transcriptional activator Cbf3 is linked to the frost-tolerance locus Fr-A2 on wheat chromosome 5A. Molecular Genetics and Genomics 269:60-67.
Remark
Trait marker Xbcd508-5A
Nearby marker on Composite map: Xbcd508
Position on Composite map estimated by GrainGenes.



Probe for Locus
S2269949
Locus
Fr-A2
S2269949
Reference
ReferenceSieber AN et al. (2016) Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat Theoretical and Applied Genetics 129:1087-1097.
Type
KASP
PCR primers
5'-FAM-tail-ACTGCAGCGAG-GATTCCCA-3'
5'-AGCCACCTCTGAGATGCCGCTT-3'
5'-HEX-tail- CTACTGCAGCGAGGATTC-CCG-3'
5'-AGCCACCTCTGAGATGCCGCTT-3'
Specificity
FAM-tail primer is specific for the frost susceptible allele of Fr-A2, while the HEX-tailed allele is specific to the frost tolerant allele