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GrainGenes Marker Report: EBmatc39

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Locus
EBmatc39
Type
Microsatellite
Chromosome
2H
Map
Barley-consensus-2003-2H105.3
Hordeum-Consensus2006-DArT-2H99.9
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Map Data
Barley consensus 2003
Barley, Consensus 2006, DArT
Probe
EBmatc39
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of EBmatc39 in the Barley, Consensus 2006 DArT maps = 2



Probe
EBmatc39
Locus
EBmatc0039
EBmatc39
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (ATC)5
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Diversity Index (DI) = 0.4
Type
PCR
SSR
PCR primers
5' TAGTCTCTTCATTTATACCATCACC 3'
5' CATGCTGATCCCCCTTCT 3'
SSR size
139
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare