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GrainGenes Marker Report: EBmatc3

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Locus	EBmatc3
Type	Microsatellite
Chromosome	5H 7H
Map	Barley-consensus-2003-5H 50.9 Hordeum-Consensus2006-DArT-7H 38
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	EBmatc3
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmatc3 in the Barley, Consensus 2006 DArT maps = 1
Probe	EBmatc3
Locus	EBmatc0003 EBmatc3
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (ATC)4N3(ATC)3 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.6
Type	PCR SSR
PCR primers	5' AATTTTGCAAAGCTGGAGG 3' 5' CATTATGGTGGGGTTCATGT 3'
SSR size	111
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare