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GrainGenes Marker Report: EBmatc28

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Probe	EBmatc28
Locus	EBmatc0028
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (ATC)3N3(ATC)6 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.59
Type	PCR SSR
PCR primers	5' AATTCCACACCCAGATGAT 3' 5' CTAGGCCTGGTGCTGAAC 3'
SSR size	93
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare