GrainGenes Marker Report: EBmatc0039
Locus
EBmatc0039
Type
Microsatellite
Chromosome
2H
Map
Barley, B73xCPI-2H 92.1 Barley-ConsensusFHB-2H 87.76 Hordeum-Consensus2007-SSR-2H 93.26 Hordeum-LxHs-2H 65
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
EBmatc0039 EBmatc39
Reference
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (ATC)5 Polymorphic Information Content (PIC) = 0.4 Developing laboratory = SCRI (R. Waugh)
Probe for Locus
EBmatc39
Locus
EBmatc0039 EBmatc39
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (ATC)5 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.4
Type
PCR SSR
PCR primers
5' TAGTCTCTTCATTTATACCATCACC 3'
5' CATGCTGATCCCCCTTCT 3'
SSR size
139
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmatc0039
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