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GrainGenes Marker Report: EBmatc0003

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Locus	EBmatc0003
Type	Microsatellite
Chromosome	5H
Map	Barley, B73xCPI-5H 128.3 Barley-ConsensusFHB-5H 129.29 Hordeum-Consensus2007-SSR-5H 127.69 Hordeum-LxHs-5H 135
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Map Data	Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmatc0003 EBmatc3
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (ATC)4N3(ATC)3 Polymorphic Information Content (PIC) = 0.6 Developing laboratory = SCRI (R. Waugh)
Probe for Locus	EBmatc3
Locus	EBmatc0003 EBmatc3
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (ATC)4N3(ATC)3 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.6
Type	PCR SSR
PCR primers	5' AATTTTGCAAAGCTGGAGG 3' 5' CATTATGGTGGGGTTCATGT 3'
SSR size	111
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare