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GrainGenes Marker Report: EBmacc0009

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Locus	EBmacc0009
Type	Microsatellite
Chromosome	4H
Map	Barley, B73xCPI-4H 56.3 Barley-ConsensusFHB-4H 69.31 Hordeum-Consensus2007-SSR-4H 58.51 Hordeum-LxHs-4H 43
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Map Data	Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmacc0009 EBmacc9
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CAA)14(CAC)7 (CAA)4 Polymorphic Information Content (PIC) = 0.47 Developing laboratory = SCRI (R. Waugh)
Probe for Locus	EBmacc9
Locus	EBmacc0009
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CAA)14(CAC)7(CAA)4 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.47
Type	PCR SSR
PCR primers	5' TCACAACAACAACAACAGCC 3' 5' AACAGCTTTGGTTGTGGGT 3'
SSR size	240
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare