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GrainGenes Marker Report: EBmac970

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Locus	EBmac970
Type	Microsatellite
Synonym	Synonym EBmac0970
Probe	EBmac970
Possible Orthologs	Bmac0970 [ Show all 6 ]
Probe	EBmac970
Locus	EBmac0970 EBmac970 EBmac970a EBmac970b
Synonym	Other name Bmac970 EBmac0970
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)8 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.49
Type	PCR SSR
PCR primers	5' ACATGTGATACCAAGGCAC 3' 5' TGCATAGATGATGTGCTTG 3'
SSR size	112
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare