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GrainGenes Marker Report: EBmac874

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Locus	EBmac874
Type	Microsatellite
Synonym	Synonym EBmac0874
Chromosome	6H
Map	Barley-consensus-2003-6H 45 Hordeum-Consensus2006-DArT-6H 71.1
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	EBmac874
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac874 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0874
Probe	EBmac874
Locus	EBmac0874 EBmac874
Synonym	Other name EBmac0874
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CA)8AA(CA)4CG(CA)8AA(CA)7AA(CA)9(TA)8 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' AACCATTCCTCACCCAGG 3' 5' GTGAATGATGTTGAGGACATTG 3'
SSR size	185
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare