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GrainGenes Marker Report: EBmac871

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Locus	EBmac871
Type	Microsatellite
Synonym	Synonym EBmac0871
Chromosome	3H
Map	Barley-consensus-2003-3H 92
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Map Data	Barley consensus 2003
Probe	EBmac871
Data Source	Langridge, Peter J.
Possible Orthologs	EBmac0871
Probe	EBmac871
Locus	EBmac0871 EBmac871
Synonym	Other name EBmac0871
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)13 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.67
Type	PCR SSR
PCR primers	5' TGCCTCTGTTGTGTTATTGT 3' 5' CCCCAAGTGAACATTGAC 3'
SSR size	180
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare