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GrainGenes Marker Report: EBmac850

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Locus	EBmac850
Type	Microsatellite
Chromosome	2H
Map	Hordeum-Zhedar2-FHB-QTL-2H 90.7
	[ Show Nearby Loci ]
QTL with Locus Marker	QFhs.dah-2H.1
Map Data	Barley, Zhedar2, FHB QTL
Probe	EBmac850
Possible Orthologs	EBmac0850
Probe	EBmac850
Locus	EBmac0850 EBmac850
Synonym	Other name EBmac0850
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)13AT(AC)10A(AC)5 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CTCAGATAACACCTTTAAACACA 3' 5' AAGACAGTTGGGTAAGCCT 3'
SSR size	132
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare