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GrainGenes Marker Report: EBmac827

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Locus	EBmac827
Type	Microsatellite
Chromosome	7H
Map	Barley-consensus-2003-7H 64.8
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Map Data	Barley consensus 2003
Probe	EBmac827
Data Source	Langridge, Peter J.
Possible Orthologs	EBmac0827
Probe	EBmac827
Locus	EBmac0827 EBmac827
Synonym	Other name EBmac0827
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CA)15TG(TA)7 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.66
Type	PCR SSR
PCR primers	5' CATGGTATTCAAACATACACG 3' 5' AAGGTCTTAAGGGGTGATG 3'
SSR size	112
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare