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GrainGenes Marker Report: EBmac824

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Locus	EBmac824
Type	Microsatellite
Synonym	Synonym EBmac0824
Chromosome	5H
Map	Hordeum-Consensus2006-DArT-5H 144.3 Hordeum-SxM-SSR-5H 169.3
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	EBmac824
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Remark	Number of populations contributing to the placement of EBmac824 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0824
Probe	EBmac824
Locus	EBmac0824 EBmac824
Synonym	Other name EBmac0824
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)4,(TG)4,(TG)4,(GT)4,(GT)4 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.43
Type	PCR SSR
PCR primers	5' GCAAGCTTCCTAAATCCTTA 3' 5' TGCAGACAGTTTTTCATATACA 3'
SSR size	308
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species	Hordeum vulgare