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GrainGenes Marker Report: EBmac806

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Locus
EBmac806
Type
Microsatellite
Synonym
SynonymEBmac0806
Chromosome
6H
Map
Barley-consensus-2003-6H56.1
Hordeum-Consensus2006-DArT-6H80.2
Hordeum-SxM-SSR-6H91.1
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Map Data
Barley consensus 2003
Barley, Consensus 2006, DArT
Barley, Steptoe x Morex, SSR
Probe
EBmac806
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
ReferenceLi JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of EBmac806 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs
EBmac0806



Probe
EBmac806
Locus
EBmac0806
EBmac806
Synonym
Other nameBmac806
EBmac0806
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (CA)4GA(CA)8,(CA)5
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.78
Type
PCR
SSR
PCR primers
5' ACTAAGTCCTTTCACGAGGA 3'
5' GTGTGTAGTAGGTGGGTACTTG 3'
SSR size
168
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare