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GrainGenes Marker Report: EBmac788

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Locus	EBmac788
Type	Microsatellite
Synonym	Synonym Ebmac0788
Chromosome	4H
Map	Barley-consensus-2003-4H 64.9 Hordeum-Consensus2006-DArT-4H 113.8
	[ Show Nearby Loci ]
QTL with Locus Marker	MQTL_Du2024.quality-4H.6
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	EBmac788
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac788 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0788
Probe	EBmac788
Locus	Ebmac0788 EBmac788
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)23 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.76
Type	PCR SSR
PCR primers	5' TAACTTACTTTATATCCATGGCA 3' 5' ATGATGAGAACTCTTCACCC 3' TAACTTACTTTATATCCATGGCA ATGATGAGAACTCTTCACCC
SSR size	168
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare