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GrainGenes Marker Report: EBmac785

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Locus	EBmac785
Type	Microsatellite
Synonym	Synonym EBmac0785
Probe	EBmac785
Possible Orthologs	EBmac0785
Probe	EBmac785
Locus	EBmac0785 EBmac785
Synonym	Other name EBmac0785
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TA)9(GT)22 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.55
Type	PCR SSR
PCR primers	5' AATTGCAAGCACTATATATATA 3' 5' ATCCAGCTAGACACATACATATT 3'
SSR size	105
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species	Hordeum vulgare