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GrainGenes Marker Report: EBmac783

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Locus	EBmac783
Type	Microsatellite
Synonym	Synonym EBmac0783
Probe	EBmac783
Possible Orthologs	EBmac0783 EBmac0783.1H EBmac0783.5H
Probe	EBmac783
Locus	EBmac0783 EBmac783
Synonym	Other name EBmac0783
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CA)5 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.12
Type	PCR SSR
PCR primers	5' TAGTACGGGGCAGTAGATAGT 3' 5' TGCATATGTGTGTACTGCTG 3'
SSR size	200
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare