GrainGenes Marker Report: EBmac764
Locus
EBmac764
Type
Microsatellite
Synonym
Synonym EBmac0764
Chromosome
7H
Map
Barley-consensus-2003-7H 77 Hordeum-Consensus2006-DArT-7H 97.7 Hordeum-GxHN-SFNB-7H 50.4 Hv-HNxH602-7H 198.2
[ Show Nearby Loci ]
Map Data
Barley consensus 2003 Barley, Consensus 2006, DArT Barley, GxHN, SFNB Barley, HN x H602, Sato2009
Species
Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe
EBmac764
Reference
Data Source
Langridge, Peter J.
Remark
Number of populations contributing to the placement of EBmac764 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs
EBmac0764
Probe
EBmac764
Locus
EBmac0764 EBmac764
Synonym
Other name EBmac0764
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (GT)16 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.84
Type
PCR SSR
PCR primers
5' AGAATCAAGATCGACCAAAC 3'
5' AAAAACATGAACCGATGAA 3'
SSR size
124
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmac764
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