Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: EBmac715

[Submit comment/correction]

Locus	EBmac715
Type	Microsatellite
Chromosome	2H
Map	Barley-consensus-2003-2H 69.4
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Steptoe x Morex, SSR
Probe	EBmac715
Data Source	Langridge, Peter J.
Possible Orthologs	EBmac0715
Probe	EBmac715
Locus	EBmac0715 EBmac715
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GT)9,(TG)6(GA)10(AAG)2(AG)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.35
Type	PCR SSR
PCR primers	5' GCGAACATTGTCATGTTAGTA 3' 5' TGTCATGCCAGACCTATG 3' GCGAACATTGTCATGTTAGTA TGTCATGCCAGACCTATG
SSR size	153
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species	Hordeum vulgare