Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: EBmac708

[Submit comment/correction]

Locus	EBmac708
Type	Microsatellite
Synonym	Synonym EBmac0708
Chromosome	3H
Map	Barley-consensus-2003-3H 116
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003
Probe	EBmac708
Data Source	Langridge, Peter J.
Possible Orthologs	EBmac0708
Probe	EBmac708
Locus	EBmac0708 EBmac708
Synonym	Other name EBmac0708
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AT)5(GT)16CT(GT)5(GC)6 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.79
Type	PCR SSR
PCR primers	5' AATTTTTGTTTCCCATGC 3' 5' AGCCCTATTGTCACAGTTTT 3'
SSR size	138
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare