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GrainGenes Marker Report: EBmac705

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Locus
EBmac705
Type
Microsatellite
Synonym
SynonymEBmac0705
Probe
EBmac705
Possible Orthologs
EBmac0705



Probe
EBmac705
Locus
EBmac0705
EBmac705
Synonym
Other nameEBmac0705
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AC)16
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.46
Type
PCR
SSR
PCR primers
5' GTGGAAAACTGAGTGAAACTC 3'
5' TTGAGGAGAAGTAATGACGAT 3'
SSR size
150
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species
Hordeum vulgare