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GrainGenes Marker Report: EBmac701

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Locus	EBmac701
Type	Microsatellite
Synonym	Synonym EBmac0701
Chromosome	4H
Map	Barley-consensus-2003-4H 37.8 Hordeum-Consensus2006-DArT-4H 112
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	EBmac701
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac701 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs	Bmac0701 Bmac701 EBmac0701
Probe	EBmac701
Locus	EBmac0701 EBmac701
Synonym	Other name Bmac701 EBmac0701
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)23 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' ATGATGAGAACTCTTCACCC 3' 5' TGGCACTAAAGCAAAAGAC 3'
SSR size	149
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare