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GrainGenes Marker Report: EBmac691

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Locus	EBmac691
Type	Microsatellite
Synonym	Synonym EBmac0691
Probe	EBmac691
Possible Orthologs	EBmac0691
Probe	EBmac691
Locus	EBmac0691 EBmac691
Synonym	Other name EBmac0691
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CA)3CG(CA)7 Quality = 1: Single product, only weak stutter bands
Type	PCR SSR
PCR primers	5' TTAACAGAGGGCATTGGT 3' 5' TCCTTTTCTCCATTTGAGTT 3'
SSR size	111
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species	Hordeum vulgare