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GrainGenes Marker Report: EBmac684

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Locus	EBmac684
Type	Microsatellite
Synonym	Synonym EBmac0684
Chromosome	2H
Map	Barley-consensus-2003-2H 65.6 Hordeum-Consensus2006-DArT-2H 77.4
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	EBmac684
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac684 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0684 [ Show all 7 ]
Probe	EBmac684
Locus	EBmac0684 EBmac684 EBmac0684b
Synonym	Other name Bmac684 EBmac0684 Ebmac0684
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TA)7(TG)11,(TG)11(TTTG)5 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' TTCCGTTGAGCTTTCATACAC 3' 5' ATTGAATCCCAACAGACACAA 3'
SSR size	172
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare