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GrainGenes Marker Report: EBmac679

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Locus	EBmac679
Type	Microsatellite
Synonym	Synonym Ebmac0679
Chromosome	4H
Map	Barley-consensus-2003-4H 45 Barley-ConsensusFHB-4H 105.5 Hordeum-Consensus2006-DArT-4H 113.5
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Consensus 2023, FHB Barley, Steptoe x Morex, SSR
Probe	EBmac679
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac679 in the Barley, Consensus 2006 DArT maps = 3
Possible Orthologs	EBmac0679
Probe	EBmac679
Locus	Ebmac0679 EBmac679
Synonym	Other name Ebmac0679 EBmac0679
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)22 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.8
Type	PCR SSR
PCR primers	5' ATTGGAGCGGATTAGGAT 3' 5' CCCTATGTCATGTAGGAGATG 3' ATTGGAGCGGATTAGGAT CCCTATGTCATGTAGGAGATG
SSR size	148
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare