Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: EBmac674

[Submit comment/correction]

Locus	EBmac674
Type	Microsatellite
Synonym	Synonym EBmac0674
Probe	EBmac674
Possible Orthologs	EBmac0674
Probe	EBmac674
Locus	EBmac0674 EBmac674
Synonym	Other name EBmac0674
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)18(AG)9 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.13
Type	PCR SSR
PCR primers	5' GAACGTATAGCAGGAGCAA 3' 5' CATCGTTCCCTTCATGAT 3'
SSR size	147
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare