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GrainGenes Marker Report: EBmac669

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Locus	EBmac669
Type	Microsatellite
Synonym	Synonym EBmac0669
Probe	EBmac669
Possible Orthologs	EBmac0669
Probe	EBmac669
Locus	EBmac0669 EBmac669
Synonym	Other name EBmac0669
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)8 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.23
Type	PCR SSR
PCR primers	5' TCTACACTACACATGTTATCTTGC 3' 5' GTGTTGATCTTCCTCCTAGTG 3'
SSR size	168
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare