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GrainGenes Marker Report: EBmac658

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Locus	EBmac658
Type	Microsatellite
Synonym	Synonym EBmac0658
Chromosome	4H
Map	Hordeum-Consensus2006-DArT-4H 96.8
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Map Data	Barley, Consensus 2006, DArT
Probe	EBmac658
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac658 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0658
Probe	EBmac658
Locus	EBmac0658 EBmac658
Synonym	Other name EBmac0658
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GT)8 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.24
Type	PCR SSR
PCR primers	5' GTATGCAAGTGTAGGTGTGTG 3' 5' CATGGGTTTACCCACATAC 3'
SSR size	198
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare