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GrainGenes Marker Report: EBmac640

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Locus	EBmac640
Type	Microsatellite
Chromosome	2H
Map	Barley-consensus-2003-2H 72.8
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Map Data	Barley consensus 2003
Probe	EBmac640
Data Source	Langridge, Peter J.
Possible Orthologs	EBmac0640
Probe	EBmac640
Locus	EBmac0640 EBmac640
Synonym	Other name EBmac0640
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GT)10,(TG)6(GA)16 Quality = 1: Single product, only weak stutter bands
Type	PCR SSR
PCR primers	5' CTCAGTGCGTTACCAGTGC 3' 5' CCTGTCATGCATAACCTATGG 3'
SSR size	176
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare