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GrainGenes Marker Report: EBmac639

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Locus	EBmac639
Type	Microsatellite
Synonym	Synonym EBmac0639
Chromosome	6H
Map	Hordeum-Consensus2006-DArT-6H 68.2
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Map Data	Barley, Consensus 2006, DArT
Probe	EBmac639
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac639 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0639
Probe	EBmac639
Locus	EBmac0639 EBmac639
Synonym	Other name EBmac0639
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)5,(TG)8 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.13
Type	PCR SSR
PCR primers	5' TTGAGGCCTACTGGTATGTAT 3' 5' TAGTCCCTCATGCACAAGT 3'
SSR size	164
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare