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GrainGenes Marker Report: EBmac615

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Locus	EBmac615
Type	Microsatellite
Synonym	Synonym EBmac0615
Chromosome	2H
Map	Hordeum-Consensus2006-DArT-2H 79.6
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Map Data	Barley, Consensus 2006, DArT
Probe	EBmac615
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac615 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0615
Probe	EBmac615
Locus	EBmac0615 EBmac615
Synonym	Other name EBmac0615
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)5CG(TG)3,(TG)10 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.23
Type	PCR SSR
PCR primers	5' AATTGGTTCGAGTCATAGCT 3' 5' CTAGTGGGTGTATGCAAGTG 3'
SSR size	173
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare