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GrainGenes Marker Report: EBmac607

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Locus	EBmac607
Type	Microsatellite
Chromosome	2H
Map	Barley-consensus-2003-2H 64.7
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Map Data	Barley consensus 2003
Probe	EBmac607
Data Source	Langridge, Peter J.
Possible Orthologs	EBmac0607 EBmac607b
Probe	EBmac607
Locus	EBmac0607 EBmac607 EBmac607b
Synonym	Other name EBmac0607
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TG)7,(TG)6(GA)10,(AG)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.38
Type	PCR SSR
PCR primers	5' GCGAACATTGTCATGTTAGTA 3' 5' AACCTTATGGATTTGGAGG 3'
SSR size	146
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare