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GrainGenes Marker Report: EBmac603

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Locus	EBmac603
Type	Microsatellite
Synonym	Synonym EBmac0603
Chromosome	7H
Map	Hordeum-Consensus2006-DArT-7H 36.5
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Map Data	Barley, Consensus 2006, DArT Barley, Igri x Franka, SSR Barley, Steptoe x Morex, SSR
Probe	EBmac603
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac603 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs	EBmac0603
Probe	EBmac603
Locus	EBmac0603 EBmac603
Synonym	Other name EBmac0603
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CA)10 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' ACCGAAACTAAATGAACTACTTCG 3' 5' TGCAAACTGTGCTATTAAGGG 3' ACCGAAACTAAATGAACTACTTCG TGCAAACTGTGCTATTAAGGG
SSR size	149
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare