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GrainGenes Marker Report: EBmac602

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Locus	EBmac602
Type	Microsatellite
Synonym	Synonym EBmac0602
Chromosome	6H
Map	Barley-consensus-2003-6H 75.5 Hordeum-Consensus2006-DArT-6H 80.2
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	EBmac602
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac602 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs	Bmac602 EBmac0602
Probe	EBmac602
Locus	EBmac0602 EBmac602
Synonym	Other name Bmac602 EBmac0602
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)9AT(AC)7(AG)9 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' GATTGGAGCTTCGGATCAC 3' 5' CCGTCTAGGGAGAGGTTCTC 3'
SSR size	205
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare