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GrainGenes Marker Report: EBmac560

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Locus	EBmac560
Type	Microsatellite
Chromosome	1H
Map	Hordeum-Consensus2006-DArT-1H 60.2
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT
Probe	EBmac560
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac560 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0560a [ Show all 7 ]
Probe	EBmac560
Locus	EBmac0560a [ Show all 7 ]
Synonym	Other name EBmac0560
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)7 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type	PCR SSR
PCR primers	5' CATATGTGTGCAAGTGTGC 3' 5' AGATCCATGAGGTTGTGC 3'
SSR size	171
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare