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GrainGenes Marker Report: EBmac557

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Locus	EBmac557
Type	Microsatellite
Chromosome	2H
Map	Hordeum-Consensus2006-DArT-2H 77.4
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT
Probe	EBmac557
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac557 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0557 EBmac0557
Probe	EBmac557
Locus	EBmac0557 EBmac557
Synonym	Other name Bmac0557
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)8 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' ATGCATGTGTAGATGTAGATGTG 3' 5' AACAAGGATAAACTAACATGGG 3'
SSR size	154
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare