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GrainGenes Marker Report: EBmac541

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Locus	EBmac541
Type	Microsatellite
Chromosome	3H
Map	Barley-consensus-2003-3H 112 Hordeum-Consensus2006-DArT-3H 148.3 Hordeum-SxM-SSR-3H 197.4
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Steptoe x Morex, SSR
Probe	EBmac541
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac541 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0541 EBmac0541
Probe	EBmac541
Locus	EBmac0541 EBmac541
Synonym	Other name EBmac0541
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)9 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.26
Type	PCR SSR
PCR primers	5' ACGGATCTACTTTAGCTAGCA 3' 5' AAACAACCCCACACAATC 3' ACGGATCTACTTTAGCTAGCA AAACAACCCCACACAATC
SSR size	106
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare