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GrainGenes Marker Report: EBmac521a

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Locus	EBmac521a
Type	Microsatellite
Chromosome	2H
Map	Hordeum-FxS-FHB-QTL-2H 79.5
	[ Show Nearby Loci ]
QTL with Locus Marker	QFhs.umn-2H.2 QHD.umn-2H.1
Map Data	Barley, FxS, FHB QTL
Probe	EBmac521
Reference	Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318.
Possible Orthologs	Bmac0521 [ Show all 6 ]
Probe for Locus	EBmac521
Locus	EBmac0521 EBmac521a EBmac521b EBmac521
Synonym	Other name EBmac0521
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)18 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' TGAAGCACAAGTGTGGAA 3' 5' AGACGTATCATTGCCCAC 3'
SSR size	163
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare