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GrainGenes Marker Report: EBmac521

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Locus
EBmac521
Type
Microsatellite
Chromosome
2H
Map
Hordeum-Zhedar2-FHB-QTL-2H102.8
[ Show Nearby Loci ]
QTL with Locus Marker
QHD.dah-2H.1
Map Data
Barley, Zhedar2, FHB QTL
Probe
EBmac521
Possible Orthologs
Bmac0521
[ Show all 6 ]



Probe
EBmac521
Locus
EBmac0521
EBmac521a
EBmac521b
EBmac521
Synonym
Other nameEBmac0521
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AC)18
Quality = 2: Single product, relatively strong stutter bands
Type
PCR
SSR
PCR primers
5' TGAAGCACAAGTGTGGAA 3'
5' AGACGTATCATTGCCCAC 3'
SSR size
163
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare