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GrainGenes Marker Report: EBmac501

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Locus	EBmac501
Type	Microsatellite
Chromosome	1H
Map	Barley-consensus-2003-1H 37.8 Hordeum-Consensus2006-DArT-1H 60.7
	[ Show Nearby Loci ]
QTL with Locus Marker	QDP.AlSl-1H QMe.SlAl-1H
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	EBmac501
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac501 in the Barley, Consensus 2006 DArT maps = 2
Possible Orthologs	Bmac0501 EBmac0501
Probe	EBmac501
Locus	EBmac0501 EBmac501
Synonym	Other name Bmac501 EBmac0501
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)13 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.93
Type	PCR SSR
PCR primers	5' ACTTAAGTGCCATGCAAAG 3' 5' AGGGACAAAAATGGCTAAG 3'
SSR size	151
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare