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GrainGenes Marker Report: EBmac415

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Locus	EBmac415
Type	Microsatellite
Synonym	Synonym EBmac0415
Chromosome	2H
Map	Barley-consensus-2003-2H 125.1 Hordeum-Consensus2006-DArT-2H 128.8 Hv-HNxH602-2H 253.1
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	EBmac415
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of EBmac415 in the Barley, Consensus 2006 DArT maps = 3
Possible Orthologs	Bmac0415 EBmac0415
Probe	EBmac415
Locus	EBmac0415 EBmac415
Synonym	Other name Bmac415 EBmac0415
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)17 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.58
Type	PCR SSR
PCR primers	5' GAAACCCATCATAGCAGC 3' 5' AAACAGCAGCAAGAGGAG 3'
SSR size	247
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare