Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: EBmac405

[Submit comment/correction]

Locus	EBmac405
Type	Microsatellite
Synonym	Synonym EBmac0405
Chromosome	1H
Map	Hordeum-Consensus2006-DArT-1H 56.2
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT
Probe	EBmac405
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of EBmac405 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	EBmac0405
Probe	EBmac405
Locus	EBmac0405 EBmac405
Synonym	Other name EBmac0405
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GT)16 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.21
Type	PCR SSR
PCR primers	5' ATGTAGCTCGGAATGTGTAGT 3' 5' CATGTTGGATAAGAGTAGAGGA 3' ATGTAGCTCGGAATGTGTAGT CATGTTGGATAAGAGTAGAGGA
SSR size	123
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare