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GrainGenes Marker Report: EBmac0874

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Locus	EBmac0874
Type	Microsatellite
Synonym	Synonym EBmac874
Chromosome	6H
Map	Barley-ConsensusFHB-6H 59.92 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 7 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0874 EBmac874
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CA)8AA(CA)4CG (CA)8AA(CA)7AA (CA)9(TA)8 Polymorphic Information Content (PIC) = 0.62 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac874
Probe for Locus	EBmac874
Locus	EBmac0874 EBmac874
Synonym	Other name EBmac0874
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CA)8AA(CA)4CG(CA)8AA(CA)7AA(CA)9(TA)8 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' AACCATTCCTCACCCAGG 3' 5' GTGAATGATGTTGAGGACATTG 3'
SSR size	185
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare