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GrainGenes Marker Report: EBmac0850

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Locus	EBmac0850
Type	Microsatellite
Chromosome	2H
Map	Barley-ConsensusFHB-2H 75.04 Hordeum-Consensus2007-SSR-2H 78.49 Hordeum-LiHs-SNP-2H 65.91 Hordeum-LxHs-2H 47
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Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Lina x H.spont, SNP Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0850 EBmac850
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AC)13AT(AC)10A (AC)5 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	EBmac850
Probe for Locus	EBmac850
Locus	EBmac0850 EBmac850
Synonym	Other name EBmac0850
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)13AT(AC)10A(AC)5 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CTCAGATAACACCTTTAAACACA 3' 5' AAGACAGTTGGGTAAGCCT 3'
SSR size	132
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare