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GrainGenes Marker Report: EBmac0849

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Locus	EBmac0849
Type	Microsatellite
Chromosome	2H
Map	Barley-ConsensusFHB-2H 73.03 Hordeum-Consensus2007-SSR-2H 67.01 Hordeum-LxHs-2H 50
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Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac0849 EBmac849
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (GT)5(TG)10TA (TG)13 Polymorphic Information Content (PIC) = 0.24 Developing laboratory = SCRI (R. Waugh)
Probe for Locus	EBmac849
Locus	EBmac0849
Synonym	Other name EBmac0849
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GT)5(TG)10TA(TG)13 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.24
Type	PCR SSR
PCR primers	5' GTTTCCGCATAAACCAAG 3' 5' ACATATCTATTGAGGGCTTGA 3'
SSR size	168
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare