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GrainGenes Marker Report: EBmac0715

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Locus	EBmac0715
Type	Microsatellite
Chromosome	2H
Map	Barley-ConsensusFHB-2H 73.03 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Barley, Cali-sib x BowmanBC, QTL [ Show all 7 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac715
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	EBmac715
Probe for Locus	EBmac715
Locus	EBmac0715 EBmac715
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GT)9,(TG)6(GA)10(AAG)2(AG)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.35
Type	PCR SSR
PCR primers	5' GCGAACATTGTCATGTTAGTA 3' 5' TGTCATGCCAGACCTATG 3' GCGAACATTGTCATGTTAGTA TGTCATGCCAGACCTATG
SSR size	153
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species	Hordeum vulgare