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GrainGenes Marker Report: EBmac0565a

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Locus	EBmac0565a
Type	Microsatellite
Chromosome	1H
Map	Barley-ConsensusFHB-1H 21.3 Hordeum-Consensus2007-SSR-1H 21.86 Hordeum-LxHs-1H 6
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Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	EBmac565
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AT)7(AC)15 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmac0565a EBmac0565b EBmac0565c
Probe for Locus	EBmac565
Locus	EBmac0565a EBmac0565b EBmac0565c
Synonym	Other name EBmac0565
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AT)7(AC)15 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' ATTTGAATGTCCAACAGAATC 3' 5' AATTGATAAGTTACTGACACACG 3'
SSR size	102
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare