GrainGenes Marker Report: EBmac0558
Locus
EBmac0558
Type
Microsatellite
Chromosome
2H
Map
Barley-ConsensusFHB-2H 73.03 Hordeum-Consensus2007-SSR-2H 69.72 Hordeum-LxHs-2H 50 Hv-Integrated2009-2H 66.9
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, LxHs
Species
Hordeum vulgare
Probe
EBmac558
Reference
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Possible Orthologs
Bmac0558
Probe for Locus
EBmac558
Locus
EBmac0558
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)7 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.18
Type
PCR SSR
PCR primers
5' GTGTGTGGTATATGTGTGTTTG 3'
5' TAGCTACACATACAAACTTGCA 3' GTGTGTGGTATATGTGTGTTTG
TAGCTACACATACAAACTTGCA
SSR size
160
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmac0558
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