GrainGenes Marker Report: EBmac0525
Locus
EBmac0525
Type
Microsatellite
Chromosome
2H
Map
Barley-ConsensusFHB-2H 73.03 Hordeum-Consensus2007-SSR-2H 70.98 Hordeum-LxHs-2H 50 Hv-Consensus2006-Marcel-2H 70.98
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
EBmac0525 EBmac525
Reference
Remark
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Oregon Wolfe Dom x Rec Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant, Lina x H. spontaneum Insert contains repeat (AC)14 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
Bmac0525 EBmac0525b
Probe for Locus
EBmac525
Locus
EBmac0525 EBmac0525b
Synonym
Other name EBmac0525
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)14 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR SSR
PCR primers
5' TGACAGTGTCTCCAGTAATGA 3'
5' GTTTGTTCTTTTGATTTTGTTG 3'
SSR size
149
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmac0525
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