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GrainGenes Marker Report: CFE181

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Probe	CFE181
Locus	cfe0181
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Zhang LY et al. (2005) High transferability of bread wheat EST-derived SSRs to other cereals Theoretical and Applied Genetics 111:677-687.
Remarks	SSR motif: (TC)8
Type	PCR SSR EST
PCR primers	AAGCCACTCTTTAATCCAGCC GTGTAGAAGGTGAGCAGGGC Left AAGCCACTCTTTAATCCAGCC Right GTGTAGAAGGTGAGCAGGGC
SSR size	218 theoretical; 234.249 observed
Amplification Conditions	Annealing temperature 59.7
Sequence	BM068627 CD900890
Linkage Group	4D
Source Species	Triticum aestivum